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Open Access Short report

Pattern of antigen expression in metastases after radioimmunotherapy of a syngeneic rat colon carcinoma utilizing the BR96 antibody

Erika Elgström1*, Sophie E Eriksson1, Tomas G Ohlsson2, Jan Tennvall13 and Rune Nilsson1

Author Affiliations

1 Department of Oncology, Lund University, Lund, Sweden

2 Department of Medical Radiation Physics, Lund University, Lund, Sweden

3 Department of Oncology, Skåne University Hospital, Lund, Sweden

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Experimental Hematology & Oncology 2012, 1:34  doi:10.1186/2162-3619-1-34

Published: 13 November 2012

Abstract

Background

Repeated administration of antibody-based therapies such as radioimmunotherapy depends on preserved antigen expression in tumor lesions. The purpose of this study was to evaluate whether the antigen expression in metastases observed after radioimmunotherapy differs from that of untreated primary tumors.

Findings

30 of the 35 Brown Norway rats with syngeneic colon carcinoma treated with 400 MBq/kg 177Lu-DOTA-BR96 exhibited consistent complete response of the primary tumor. 13 animals developed metastases that were detected after treatment. The antigen expression was reduced in 17 of 23 metastases detected after radioimmunotherapy compared with untreated tumors. No tumors completely lacked positively stained tumor cells.

Conclusions

Although it was not possible to demonstrate that the antigen reduction is triggered by the radioimmunotherapy this result stress the importance of considering the risk of reduced antigen expression in metastases after radioimmunotherapy prior to further targeted therapies.

Keywords:
Radioimmunotherapy; Rat colon carcinoma; 177Lu; Antigen expression; Metastasis