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Open Access Letter to the Editor

Bone marrow non-mesenchymal mononuclear cells induce functional differentiation of neuroblastoma cells

Chareerut Phruksaniyom1, Permphan Dharmasaroja1* and Surapol Issaragrisil2

Author Affiliations

1 Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand

2 Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand

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Experimental Hematology & Oncology 2013, 2:9  doi:10.1186/2162-3619-2-9

Published: 3 April 2013


Less is known about the non-mesenchymal mononuclear cell fraction of human bone marrow on functional adaptation of neuroblastoma cells. Using immunocytochemistry, we showed that bone-marrow mononuclear cell (BMMC)-conditioned medium can induce tyrosine hydroxylase expression in neuroblastoma cells, which is similar to the effect of retinoic acid. Using quantitative RT-PCR, we showed that NGF, CNTF, and BDNF mRNAs were detected in unfractionated BMMC populations from all human donors at different expression levels. Our results suggest that cells of the non-mesenchymal mononuclear cell fraction can induce functional adaptation of neuroblastoma cells, probably via their secreted trophic factors.

Bone-marrow mononuclear cells; Neuroblastoma cells; Tyrosine hydroxylase; Trophic factors; Neuronal differentiation